HiPerFect Transfection Reagent有助于siRNA的高效吸收，实现了低浓度下的基因沉默，而不损害knockdown的效率。 Q：在siRNA转染过程中为何应当避免细胞毒性？ A：siRNA导入所引起的细胞毒性干扰了RNAi后的数据分析，因为表型分析不再可靠。

产品名： The Silencer siRNA Transfection Kit: siPORT™ Amine 和 siPORT™ Lipid 厂家：Ambion 优点：专为siRNA转染不同细胞类型而设计. 简介：目前 主流的转染试剂类型主要是脂质类和多胺类两种，由于 不同的细胞“偏爱”不同类型的转染试剂，因而对不同的细胞株的转染效率可能大相径庭，另外转染效率也和被转 ...

Cy3-labeled siRNA transfection into inner ear in chinchilla through round-window permeation with or without TAT-DRBD. To further study the specific kinetic profiles of Cy3-labeled siRNA entering ...

Polyplus-transfection has announced the launch of jetPRIME ™, a new reagent for DNA and siRNA transfection. jetPRIME™ brings pharmaceutical and biotechnology researchers a number of advantages for day ...

High-transfection efficiency and a robust and reliable protocol that works for most cell lines is important and become paramount for siRNA screening programs. Transfection efficiency has to be ...

Achieve high-efficiency siRNA/miRNA transfection with INTERFERin®. Achieve high-efficiency siRNA/miRNA transfection with INTERFERin®. Skip to content. Menu. Medical Home Life Sciences Home.

Image: Courtesy of Ambion SILENCE! HeLa cells transfected with Cy3-labeled GAPDH or Scrambled siRNAs using Ambion's siPORT Lipid Transfection Agent were harvested 48 hours post-transfection. Red: ...

Avoid fluorescent siRNA controls when working at low-siRNA concentration since high-siRNA concentration is required for detection (20–50 nM). Follow up the transfection reagent protocol.

Introduction. siRNA is a short (typically 20–27 bp) dsRNA molecule that can be endogenously found in a cell or introduced from exogenous sources (e.g., synthetic siRNAs) by transfection. siRNAs ...