This CRISPR-Cas9 delivery reagent is formulated specifically to deliver large Cas9/sgRNA expression vectors at up to 10x higher efficiency than competitor reagents into hard-to-transfect primary cells ...
Xfect RNA转染试剂可用于高效转染多种类型RNA至哺乳动物细胞，是包含转染所需要全部试剂的完整系统。转染信使RNA可实现快速基因表达，排除DNA转染所引起的基因组整合。大多数转染试剂对细胞 ...
在该实验中，小鼠原代 T 细胞共转染了 Cas9 mRNA、目的基因 sgRNA 和 EGFP-mRNA（其中EGFP mRNA用于目标细胞的筛选），成功实现了以下效果： 1、高转染 ...
Hosted on MSN1mon
New Class of CRISPR-Cas Enzymes for Precise Genome EditingClass II Cas9 and Class V Cas12 are currently widely ... amino acid information related to hydrogen bonding with target DNA-sgRNA. The GFP reporting system was then used to test the specificity ...
基因敲入所面临的主要难题是修复模板的制备和如何与Cas9-sgRNA核糖核蛋白复合物共导入至细胞。虽然单链DNA（ssDNA）修复模板展示出了优于双链DNA（dsDNA）模板的众多优势，但是其制备难度和 ...
Some results have been hidden because they may be inaccessible to you
Show inaccessible results
Feedback