Initial qPCR cycles with minimal amplification and relatively low fluorescent signals form a baseline for analysis. At a specific cycle number, known as the threshold cycle (Ct), the fluorescent ...

T o: Initial number of target gene copies; R o: Initial number of standard gene copies; E: Efficiency of amplification; ct,t: Threshold cycle of target gene; ct,r: Threshold cycle of standard gene ...

The UltraMarathonRT ® RT-qPCR Kit dramatically improves data reliability in quantitative PCR by maintaining consistent cycle threshold (Ct) values, regardless of RT primer type, RNA complexity or ...