qPCR, also known as real-time PCR, is an extension of traditional PCR ... The primer ends should be GC-rich to allow efficient template binding and high melting temperatures. Design primers with 40-60 ...

3). Figure 3: ABsolute Fast QPCR Master Mixes deliver accurate and highly reproducible QRT-PCR data across a large template dynamic range. Ginzinger, D. Gene quantification using real-time ...