She is passionate about making complex scientific concepts accessible to readers. View Full Profile. Learn about our Editorial Policies. Quantitative polymerase chain reaction (qPCR) is a molecular ...

Until this past January, few outside academic and diagnostic labs knew what quantitative reverse-transcription PCR (RT-qPCR) was, much less its role in viral and bacterial disease detection.

The ubiquity of quantitative PCR (qPCR) for interrogating gene expression in a variety of research and clinical contexts makes it an indispensable laboratory tool. Among the most critical variables ...