荧光定量PCR（qPCR）是目前病原检测领域使用最为广泛的核酸检测方法。尤其是非洲猪瘟和新冠发生后，qPCR检测得到了极大的普及，对于刚刚进入qPCR ...

分子POCT“样本进、结果出”的特点，不仅直接降低了核酸检测的门槛，打破核酸检测“四套间”的桎梏，利好qPCR检测的微型化及便携化。 实现分子 ...

而数字PCR本身就是绝对定量的，不需要做标准曲线。另一方面，qPCR技术更为成熟名头也更响，市面上有大量的商业化产品可供选择。dPCR目前还是个小鲜肉，应用没有qPCR那么广泛，价格也更昂贵一些。与dPCR相比，qPCR更适合高通量分析，而且动态范围很宽。

为解决布鲁氏菌病（Brucellosis）疫苗免疫抗体与自然感染抗体难以区分、疫苗株和自然强毒株鉴别困难的问题，研究人员开展了布鲁氏菌 S2 疫苗株差异诊断方法的研究。成功建立了 duplex qPCR 检测方法，该方法特异性、敏感性和重复性良好，可用于临床布鲁氏菌病的鉴别诊断，为疫病防控提供技术支持。

Like PCR, qPCR amplifies DNA using a DNA polymerase enzyme.This adds new nucleotides to the 3’-OH end of a short, single-stranded DNA segment, called a primer, which complements the template strand. 2 ...

qPCR for these model samples were carried out using EEF1G primer set and MGB fluorogenic probe listed in Supplementary Table 1 by measuring the fluorescence intensities during thermal cycling ...

qPCR is a technique for the selective amplification and quantitative detection of regions of DNA or complimentary DNA (cDNA). Oligonucleotide primers flanking a region of interest are used to amplify ...

Conclusions: Our findings validate the robustness of the NGS model and demonstrate the feasibility of transferring NGS-identified biomarkers to qPCR, a cost-effective and scalable diagnostic method.

Here, we describe a simple test for detection of SARS-CoV-2. The sensitivity of this test is similar to that of reverse-transcription–quantitative polymerase-chain-reaction (RT-qPCR) assays.