The resulting amplification curve shows when targets are amplified ... The addition of a reverse transcription (RT) step to qPCR occurs when the target is RNA, such as when testing for the ...

With the ever-growing popularity of quantitative PCR (QPCR) as a sensitive technique for quantifying DNA and RNA, there is increasing demand for higher throughput of reactions and faster protocol ...

Example Files are available to download above ("ddCT_Example.txt" and "PI_Example.txt"). These files represent example setups of qPCR data and can be run using qPCRTools. Ct values are made up and may ...

She is passionate about making complex scientific concepts accessible to readers. View Full Profile. Learn about our Editorial Policies. Quantitative polymerase chain reaction (qPCR) is a molecular ...

The ubiquity of quantitative PCR (qPCR) for interrogating gene expression in a variety of research and clinical contexts makes it an indispensable laboratory tool. Among the most critical variables ...