A western blot is a laboratory method used ... The membrane is then incubated with an antibody called the primary antibody, which specifically binds to the protein of interest.

Essentially, western blotting has three main elements: separate proteins in the sample by size, transfer to a solid support and visualize the target protein using primary and secondary antibodies. The ...

Western blotting allows scientists to identify specific proteins ... before and during immunoblotting helps prevent nonspecific antibody binding on the membrane. 6 The primary antibody, specific to ...

Directly-labeled primary antibodies also allow researchers to multiplex using ... Never assume that antibodies validated for one application, such as western blotting, will work well in another, such ...

high-throughput method for validating the target-specificity of antibodies for the application of western blot. View the full article Also, find out more about how the ENCODE project – an NIH-funded ...

A western blot is a laboratory method used ... The membrane is then incubated with an antibody called the primary antibody, which specifically binds to the protein of interest.