Qdot Western Blotting Kits are designed to be a 'plug-and-play' substitute for colorimetric or chemiluminescent detection systems on blots labeled with rabbit and mouse primary antibodies.

A western blot is a laboratory method used ... The membrane is then incubated with an antibody called the primary antibody, which specifically binds to the protein of interest.

Essentially, western blotting has three main elements: separate proteins in the sample by size, transfer to a solid support and visualize the target protein using primary and secondary antibodies. The ...

The main steps in a western blot protocol are as follows. Sample preparation, including protein extraction and quantification Protein separation via gel electrophoresis Membrane transfer of proteins ...

high-throughput method for validating the target-specificity of antibodies for the application of western blot. View the full article Also, find out more about how the ENCODE project – an NIH-funded ...