This study presents an enhanced nuclease prime editor (uPEn) that significantly improves gene knock-in and knockout ...

Rapidly add 300 μl of ice-cold nuclease S1 digestion buffer and immediately remove the tube from the water bath. Vortex gently to mix and then transfer the tube to a water bath set at the ...

1| Pellet ∼10 8 cells at 300g at 4 °C for 10 min in a centrifuge. The nuclei should be kept cold throughout the procedure. Manipulations and transport to the centrifuge should be done on ice. 2 ...

The team observed a 4.4-fold increase in the editing efficiency of this modified nuclease, surpassing most of the commonly used RNA-guided endonucleases, including CjCas9. They called this variant ...

To uncover the mechanisms for nuclease regulation, we apply a multidisciplinary approach that includes hybrid structural biology, biochemistry, and cell biology. Together our work aims to understand ...

The complex adopts a more compact structure when bound to double-stranded DNA (dsDNA), with the target DNA strand making a pronounced U-turn and interacting with the HNH nuclease domain.

This led to the identification of AcrVIB1, the first anti-CRISPR protein targeting the Cas13b nuclease. The nuclease Cas13b can recognize and cut RNA. It is currently used to silence genes ...

This led to the identification of AcrVIB1, the first anti-CRISPR protein targeting the Cas13b nuclease,” says HIRI department head Prof. Chase Beisel, who led the study together with the ...

The amount of remaining host cell DNA was significantly reduced by nuclease treatment of the lentiviral supernatant. qPCR was used to quantify the DNA of residual host cells. Image Credit ...