This study presents an enhanced nuclease prime editor (uPEn) that significantly improves gene knock-in and knockout ...

Rapidly add 300 μl of ice-cold nuclease S1 digestion buffer and immediately remove the tube from the water bath. Vortex gently to mix and then transfer the tube to a water bath set at the ...

The team observed a 4.4-fold increase in the editing efficiency of this modified nuclease, surpassing most of the commonly used RNA-guided endonucleases, including CjCas9. They called this variant ...

For its wholly owned in vivo programs, Precision is developing an ARCUS nuclease that targets chronic hepatitis B covalently closed circular DNA. For its primary hyperoxaluria type 1 program ...

The amount of remaining host cell DNA was significantly reduced by nuclease treatment of the lentiviral supernatant. qPCR was used to quantify the DNA of residual host cells. Image Credit ...

To uncover the mechanisms for nuclease regulation, we apply a multidisciplinary approach that includes hybrid structural biology, biochemistry, and cell biology. Together our work aims to understand ...

This led to the identification of AcrVIB1, the first anti-CRISPR protein targeting the Cas13b nuclease. The nuclease Cas13b can recognize and cut RNA. It is currently used to silence genes ...

This led to the identification of AcrVIB1, the first anti-CRISPR protein targeting the Cas13b nuclease,” says HIRI department head Prof. Chase Beisel, who led the study together with the ...

The complex adopts a more compact structure when bound to double-stranded DNA (dsDNA), with the target DNA strand making a pronounced U-turn and interacting with the HNH nuclease domain.