The presence of even trace amounts of EDTA will result in failure of the restriction endonuclease digestion. Remove the enzyme mixture from the tube and, using a sterile loop, gently lift the ...

Researchers have found a way to study the endonuclease-driven digestion of drug-loaded DNA nanostructures in real time. As the team investigated the binding of Dox to the DNA structures in great ...

10 U/mL of endonuclease for partial host cell DNA digestion, or 100 U/mL of endonuclease for full digestion. When producing rAAV8 and rAAV9 in HEK293 cell cultures, they report achieving yields ...

have found a way to study the endonuclease-driven digestion of drug-loaded DNA nanostructures in real time. The researchers' previous experiments used high-speed atomic force microscopy to show ...

The yeast nuclear endonuclease Rnt1 has a well-characterized role in the maturation of many ncRNA precursors. However, the scope and consequence of its function in mRNA degradation pathways is much ...

“In addition, we introduced a selective precipitation step for host cell DNA before proceeding with endonuclease digestion. This additional step allows us to get rid of host cell DNA very ...

PATIENTS AND METHODS: One hundred sixteen patients with MTC verified by postoperative pathologic examination were subjected to genetic analysis of RET exons 10, 11, 13, 14, and 16 by means of ...

the greater the number of fragments generated from digestion. For example, if there is a short sequence of GAGC that occurs repeatedly in a sample of DNA. The restriction endonuclease that ...

Despite the degradation step, as achieved by the endonuclease, PP residues remain as a result of incomplete digestion. To date, no chromatographic methods for mcDNA analysis have been developed ...