The incubation was terminated by the addition of an equal volume of saturated 2,4-dinitrophenylhydrazine (DNPH) in 2 N HCl. The reaction mixture was centrifuged to remove precipitated protein and ...

8 Among potential byproducts of ROS with proteins, plasma 2,4-dinitrophenylhydrazine (DNPH) reactive carbonyl derivatives (RCD) and protein thiol groups (P-SH) have been proven as tools in ...