After 24 h, cells were transfected with 250 ng/well of luciferase reporter plasmid, 10 ng/well of pRL-TK (Promega) and 250 ng/well of each nuclear receptor expression plasmid and/or blank ...

Total amounts of transfected DNA were kept constant by supplementing with control empty expression vector plasmids as needed. All cells were co-transfected with pRL-TK (Promega) as a normalization ...

MURRYSVILLE, Pa. – A 16-year-old boy with a "blank expression" stabbed and slashed 19 students and a police officer in the crowded halls of his suburban Pittsburgh high school Wednesday before ...