Researchers design novel microfluidic module for controlling the porosity of manufactured materials
and Water-in-Oil (W/O) emulsions with changes in droplet size and volume fraction while maintaining a constant extrusion rate ...
Nature3mon
Amplification of complex gene libraries by emulsion PCR
After the addition is complete, continue stirring for 5 min. A w/o emulsion is generated containing approximately 10 8 –10 9 PCR-competent compartments per milliliter of emulsion. The ...
Nature1mon
BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions
Binding buffer: 5 mM Tris-HCl (pH 7.5), 0.5 mM EDTA, 1 M NaCl Breaking buffer: 10 mM Tris-HCl (pH 7.5), 1% Triton-X 100, 1% SDS, 100 mM NaCl, 1 mM EDTA Primers 1 and 2 are used for ...