Membranes were blocked in TRIS-buffered saline with the use of 5 percent milk and 2 percent bovine serum albumin. The membranes were incubated overnight with 1 μg of a rabbit polyclonal antibody ...

Blots were fixed for 30 minutes with 0.5 percent glutaraldehyde in TRIS-buffered saline (500 mM sodium chloride and 20 mM TRIS, pH 7.5), blocked for 30 minutes in 0.75 percent Blotto (nonfat ...

Every molecular biologist knows about Tris, but few have questioned its suitability as an electrophoretic buffer component. An intrepid pair of researchers at Johns Hopkins' Sidney Kimmel ...