But as with other transfection methods, the optimal conditions for electroporation of untested cell lines must be determined experimentally. 1| Collect the cells to be transfected from cultures in ...

1| Harvest exponentially growing cells 24 h before transfection by trypsinization and replate them at a density of 1 × 10 5 to 4 × 10 5 cells/cm 2 in 60-mm tissue culture dishes or in 12-well ...