Investing in early process development is crucial. A potential bottleneck is transfection, where genetic material is introduced into the host cells, leading to the production of viruses.
We tested this system using 30-ml cultures in shake flasks and were able to scale up the production process using the same transfection procedure irrespective of the final culture volume (5-liter ...
The lack of lipid-based transfection products that efficiently transfect these cell types has challenged researchers for many years. To overcome these obstacles, Lipofectamine 3000 is designed to ...