Electroporation works well with cell lines that are refractive to other techniques, such as calcium phosphate–DNA coprecipitation. But as with other transfection methods, the optimal conditions ...

Change the medium 1 h before transfection. 2| To prepare the calcium phosphate–DNA coprecipitate, combine 100 μl of 2.5 M CaCl 2 with 25 μg of plasmid DNA in a sterile 5-ml plastic tube and ...

The most recent and sophisticated multi-component transfection reagent, FuGENE ® 4K, was created to transfer DNA into both difficult and routine mammalian cell lines. Users are able to obtain ...

操作步骤简单：简单易用的操作步骤，可进行反向转染 (Reverse Transfection)，无需复杂优化。 在转染前一天铺细胞，在转染当天 细胞融合度在75%左右。 转染当天，将以无血清培养基稀释 DNA。再加入适量ViaFect™ 转染试 剂与DNA构成适当比例，孵育 5-20min。 加入适量 ...

Reporter genes are generally joined to a regulatory DNA sequence in an expression vector that is usually propagated in Escherichia coli before transfection into the cell type of interest. 1 A control ...