Researchers working with RNA-seq face a major challenge: ribosomal RNA (rRNA). While rRNA can comprise up to 90% of a total RNA sample, in most cases, it doesn’t provide information about the ...

单细胞测序技术经过了10余年的发展，取得了众多的技术进展与突破。其中，单细胞RNA测序技术中，Smart-Seq方法以其可实现完整mRNA扩增的优势，被广泛的应用。而近期广受关注的另外一个高通量单细胞RNA测序技术由Macosko E团队开发，其研究成果于2015年发表在 ...

The Genomic and RNA Profiling core offers various RNA-seq chemistries for many sample types. Total RNA-seq: Capture of both coding and non-coding RNAs. Compatible with degraded RNA. Full-length ...

为解决儿童 B-ALL 复发预测难题，南方医科大学南方医院研究人员整合 RNA-seq 数据开展相关研究。他们构建了 PCD 相关的复发预测模型，还明确多种 PCD 模式与复发的关系。该成果对疾病研究及个性化治疗意义重大，值得科研读者一读。 南方医科大学南方医院儿科 ...

In total RNA sequencing experiments, many reads are not necessarily useful or desired for gene expression studies, including intronic reads from pre-mRNA or noncoding transcripts. Using target ...

Cogenics has launched the 454 Genome Sequencer FLX system at its facility in Meylan, Franceto offer next generation sequencing services. GATC plans to use the Genome Sequencer to offer its customers ...

This mismatch can overemphasize cell types with high total gene expression and underemphasize those with low expression in the bulk RNA-seq deconvolution. Similar issues arise from differences in ...