The titration is performed at constant pressure and temperature, meaning that a single ITC experiment offers data on the binding enthalpy, the equilibrium association constant and the stoichiometry, ...

As shown in Figure 2, the relative magnitude of the equilibrium constant for a reaction with a 1:1 stoichiometry affects the shape of the titration curve, as does the concentrations of the ...

Also, knowing the concentrations of the two components, for instance protein and ligand, makes it possible to determine the stoichiometry of the binding reaction. If the titration is performed at ...

Karl Fischer discovered that if sulfur dioxide is added in the excess the same reaction can be used for water determination by titration of the produced acids. His base of choice was pyridine which ...

Along with that, we saw that the MW trace reached a complex with 2:1 Nap1:H2A-H2B stoichiometry. Upon further titration, beyond an equimolar amount (1.5 and 2.0 molar equivalents), the MW trace ...

Automated washing with detergent of the sample cell and titration syringe assists in producing high quality reproducible data. • All binding parameters (affinity, stoichiometry, enthalpy and entropy) ...