For example, repression of the lac operon by its repressor, called lacI, is inhibited by the ligand allolactose, to which the repressor protein directly binds. Thus, lactose, from which ...

So Collins and his graduate student Tara Deans decided to follow the 'divide and conquer' paradigm; they combined repression at a lactose operon (lac) and a tet-controlled promoter with RNA ...