Engineering the replication of target DNA through cloning, or changing its genetic code through mutations, are detail-oriented processes whose foibles can spell disaster. Caitlin Smith looks at ...

Without this first step, no DNA can be replicated. In this way, helicases are machines or, because of their size, nanomachines. If helicases are nanomachines, then ‘ATP’, or adenosine ...

In step 2, the gene fragment is integrated into a circular piece of DNA, called a plasmid, which is the main delivery vehicle into the cell and contains an antibiotic resistance gene for plasmid ...

Figure 2 shows the configuration of a sample template DNA oligonucleotide derived from the RNA oligonucleotide design. Synthesize or order the template oligonucleotide designed in steps 1 and 2 ...