During the past few years a novel approach based on the use of type IIS restriction enzymes in combination with T4 DNA ligase has become popular (known as Golden Gate cloning, PLoS ONE 3 ...

Use any standard cloning method to clone the PCR product from Step 13 into any vector that can be used for DNA sequencing (e.g., pCAMBIA 1300 if the DNA is larger than 3.0 kb or pUC118 for shorter ...

Custom DNA primer synthesis is available ... 1–3 h depending on the complexity of the inserted library(s) Steps 15–23, Transformation of the cloning reaction (e.g., for the expression and ...

New England Biolabs (NEB®) announces the introduction of the NEBuilder HiFi DNA Assembly Cloning Kit and Master Mix for one-step cloning and multiple DNA fragment assembly in as little as 15 minutes.

Therapeutic cloning, by contrast, requires insertion of DNA into a human egg from which ... sounds controversial because it is. It stops one step, albeit a critical step, short of reproductive ...

Despite advancements in DNA sequencing (reading), DNA synthesis (writing) has historically not kept pace. This has created a discrepancy of 3,000 times more DNA sequenced versus constructed ...

The BioXp® System is a key part of our process, enabling us to reduce timelines, eliminate manual steps, and more broadly test and iterate. We expect the new Select DNA Cloning kit to be of great ...