参选项目：检测小分子量核酸的高效Southern Blotting 背景：目前，尽管PCR的方法可以检测到基因组的改变，却不能够如实提供转基因拷贝数的变化，因此还不能够完全替代Southern Blotting。经典的Southern Blotting方法，仍然是对转基因和基因组的动态变化进行检测的关键 ...

Blotting methods are an adjunct to gel electrophoresis, a method for separating DNA 1, RNA 2 and proteins 3 with exceptional resolving power. They allow the detection of specific molecules among ...

一提起Western blot，大家会不禁联想到Southern blot和Northern blot。其实它们的名字也是个有趣的故事。1975年，Edwin Southern教授发明了DNA杂交检测技术，故命名为Southern blot。之后，James Alwine、David Kemp和George Stark三位教授又发明了RNA杂交检测技术，因与Southern blot相似 ...

The Southern blot is the most common way to analyse restriction endonuclease-digested DNA to find specific sequences of interest. It uses the fact that denatured nucleic acids will bind to ...

This protocol describes the first stages of Southern blotting: digestion of genomic DNA with restriction enzymes, separation of the resulting fragments by gel electrophoresis, and capillary ...

Western blotting was named in a nod to a tradition that had been inadvertently started when Edwin Southern penned his new invention ‘the Southern blot’. For the latest information on western blotting, ...

There are several types of blotting techniques based on the target molecule that each of the technique is used to detect. Dr. Edwin Southern developed one of the first blotting techniques called ...

The technique was developed in 1979 1 by Harry Towbin and colleagues and later named the “western blot” due to the technique’s similarity to Southern blotting. 2 Briefly, proteins in an aqueous sample ...

The first blotting technique developed by Dr. Edwin Southern is called the Southern blot. This technique was subsequently modified so as to detect other target molecules. These techniques being ...