Enzymes are highly specific biocatalysts, yet they can promote unwanted side reactions. Here we investigated the factors that direct catalysis in the enoyl-thioester reductase Etr1p. We show that ...

For example, N-glycosylation (attachment of glycans to nitrogen on the amine side chain of asparagine) and O-glycosylation (attachment of glycans to oxygen on serine and/or threonine).