In a sequencing reaction, a single-stranded DNA fragment is combined with the appropriate sequencing primer; a ddNTP (for example, ddTTP); and the normal dNTPs (dTTP, dCTP, dATP and dGTP), one of ...

For instance, if the lowest band across all four lanes appears in the A reaction lane, then the first nucleotide in the sequence is A. Then if the next band from bottom to top appears in the T ...

The quality of your sequencing template greatly affects the success of the sequencing reaction. Sequencing by capillary electrophoresis is highly sensitive to sample contamination. Potential ...

A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...