荧光定量PCR仪的光学系统负责捕捉PCR反应中的荧光信号。赛默飞7500采用了多种荧光检测技术，包括激光激发、光谱分光和荧光探测等。光学系统可以准确地监控不同荧光染料或探针的信号，进行实时检测。其光源主要采用高效的激光光源，能够提供高强度的激发光，确 ...

We have developed a two-step, real-time, quantitative assay, using the polymerase chain reaction with reverse transcription (RT-PCR) and based on SYBR Green I monitoring of PCR product ...

The second class consists of dyes that bind double-stranded DNA and become fluorescent; the most commonly used of these is SYBR Green I, sold by many companies that provide PCR reagents.

绝对定量PCR、相对定量PCR、Sybr Green定量及熔解曲线分析、SNP分析、Molecular Beacon熔解曲线分析等 使用简便：实验条件设置、反应过程监控、数据显示分析三大功能模块从同一界面链接，直观简便 实时观测和数据分析：反应过程中可实时监测和收集荧光信号 ...