As a result, researchers wanting to use real-time PCR are faced ... for real-time quantitative analysis of RNA called linear-after-the-exponential (LATE)-PCR 5. “When using real-time PCR to ...

So the question remains "What are the essential prerequisites for establishing a quantitative RT-PCR assay ... allows for statistical analysis of differences in PCR product yield.

or real-time RT–PCR. Fast and accurate, real-time RT–PCR has been critical in detecting, tracking and studying COVID-19. But what is it, and how does it work? Let us explain.

This technique allows scientists to see the results almost immediately while the process is still ongoing, whereas conventional RT–PCR only provides results at the end of the process. Real time RT–PCR ...

Variation in gene expression among cell types, tissues, and organisms is commonly examined by reverse transcription quantitative PCR, or RT-qPCR. In this process, RNA is isolated from samples of ...