免疫反应基因表达分析中数据标准化单一标准的选择问题尚未解决。研究人员选取HPRT1、SDHA等为候选参考基因，开发多重实时 RT-PCR 系统分析。用相关算法构建稳定指数，用于 TLRs 基因表达水平归一化，证实该系统适用于免疫反应基因表达分析。

You have full access to this article via your institution. So the question remains "What are the essential prerequisites for establishing a quantitative RT-PCR assay?" To date, a great deal of ...

or real-time RT–PCR. Fast and accurate, real-time RT–PCR has been critical in detecting, tracking and studying COVID-19. But what is it, and how does it work? Let us explain.

Real-time PCR is the favored method for measuring gene expression. Researchers benefit from a vast and growing choice of reagents and instruments for their experiments. Laura Bonetta reports.