Reconstructing full-length transcripts from high-throughput RNA sequencing data is difficult without a reference genome sequence. Grabherr et al. describe Trinity, an algorithm for assembling full ...

Explore the fundamentals of RNA-seq, its applications in gene expression analysis and a step-by-step protocol. ... as are quality control tools like Picard or Qualimap. 13 De novo assembly will allow ...

However, RNA-seq can only gather fragments of mRNAs, rather than full-length molecules. ... While accurate meta-assembly has not yet been achieved with current computational models, ...

Here, we highlight a few applications of full-length RNA sequencing to better understand plant and animal genomes and the workflow for isoform sequencing methods (Figure 1). Figure 1.

PolyA-RNA-seq: Capture of coding RNAs. Requires intact RNA. Full-length transcripts are captured. Recommended read depth is 15-30 million reads/sample although deeper sequencing may be required in ...

BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing. Genome Biology , 2019; 20 (1) DOI: 10.1186/s13059-019-1671-x Cite This Page : ...

Long-read sequencing technology, compared to widely-used short-read sequencing technology, can improve mapping accuracy, genome assembly, and structural variation detection.

New RNA-seq, metabolomics protocol offers more efficient extraction that maintains data integrity. ScienceDaily . Retrieved June 2, 2025 from www.sciencedaily.com / releases / 2023 / 05 ...