Hybridize the DNA and RNA at the chosen temperature for 12–16 h. 13. Keeping the body of the tube submerged, open the lid of the hybridization tube. Rapidly add 300 μl of ice-cold nuclease S1 ...

Our laboratory carried out research in two areas. In one, we studied the structure and mechanism of activity of the catalytic RNA moiety of the RNA processing enzyme ribo-nuclease P (Rnase P). In the ...

The nuclease Cas13b can recognize and cut RNA. It is currently used to silence genes, whether to study their function, clear viruses or counteract genetic diseases linked to the gene.” ...

We have solved the cryo-EM structure of AcrVIA1 bound to Cas13a crRNA, establishing that AcrVIA1 interacts with the guide-exposed face of Cas13a, thereby preventing access to the target RNA and the ...