Hybridize the DNA and RNA at the chosen temperature for 12–16 h. 13. Keeping the body of the tube submerged, open the lid of the hybridization tube. Rapidly add 300 μl of ice-cold nuclease S1 ...

The team observed a 4.4-fold increase in the editing efficiency of this modified nuclease, surpassing most of the commonly used RNA-guided endonucleases, including CjCas9. They called this variant ...

Wulf Blankenfeldt at HZI. "The nuclease Cas13b can recognize and cut RNA. It is currently used to silence genes, whether to study their function, clear viruses, or counteract genetic diseases ...