研究人员为开展此项研究，运用了多种关键技术方法。在化合物合成方面，采用常规及微波辅助无溶剂法；利用 MTT 法（一种基于活细胞线粒体中的琥珀酸脱氢酶能使外源性 MTT 还原为水不溶性的蓝紫色结晶甲瓒并沉积在细胞中，而死细胞无此功能的原理，来检测细胞存活和生长的方法）评估细胞毒性；通过分子对接模拟研究化合物与 B - Raf 激酶的相互作用；借助 BOILED - Egg ...

is attributed to destabilization of the Raf-1 protein, and to the resulting inhibition of mitogen-activated protein kinase (MAPK). GA triggers a rapid and marked decrease in the kinase activity of ...

Aberrant activation of the Ras/Raf/MAPK/ERK kinase/extracellular signal-regulated kinase cascade and the VEGF pathway are commonly observed in breast cancer cells leading to malignant cell ...

Black Diamond Therapeutics has found a partner for its deprioritized solid tumor candidate. Five months after cutting support ...

of the mitogen-activated protein kinase (MAPK) pathway, IMM-6-415, will be evaluated as an oral, twice-daily treatment. Patients with rapidly accelerated fibrosarcoma (RAF), kirsten rat sarcoma ...