The cytostatic and cytotoxic effects of doxorubicin on the cells were assessed with MTT assay as described ... cells was demonstrated by annexin V and propidium iodide staining (Fig.

In normal cells, phosphatidylserine (PS) residues are found in the inner membrane of the cytoplasmic membrane. During apoptosis, the PS residues are translocated in the membrane and are externalised.

Cell apoptosis was detected by flow cytometry with Alexa Fluor 488 annexin V and propidium iodide fluorescence staining (Molecular Probes, Eugene, Oregon).