Plasmid DNA (35 μg in 25 μl PBS) encoding firefly luciferase (pCMV ... were collected and processed for luciferase activity using a commercial assay (Promega, Madison, Wisconsin).

Inspired by these elegant natural mechanisms, the research team developed a reporter molecule based on firefly luciferase (FLuc) that operates in a similar manner. Luciferase is a bioluminescence ...