“I then purified this plasmid cloning vector that had the gal operon and showed that I could take the purified DNA and reintroduce it back into E. coli using a method that [Stanford biochemist] Dale ...

One particular plasmid design will not suit all needs. The pSM2 vector therefore is constructed ... of the template used in amplification and cloning. Solution: The biggest problem that we have ...

To prepare the cloning vectors, transform separately pET11a-link-NGFP and pMRBAD-link-CGFP into an E. coli strain that gives high-quality plasmid DNA ... Purify the vector products by ...

This cassette is then transferred to the adenovirus vector plasmid (pAdeno X, Clontech Laboratories or pAd5F35 ... Production of adenovirus vectors can begin at 1 of 3 steps. Service can be initiated ...