called ester bonds, between the sugar base of one nucleotide and the phosphate group of the adjacent nucleotide. The sugar is the 3' end, and the phosphate is the 5' end of each nucleiotide.

Figure 1. Hydrolysis of an ester consists of water adding to the ester bond and displacing an alcohol (R-OH), forming a carboxylic acid. The red boxes show the original water molecule. The blue hatch ...

Where the phosphate group proximal to the nucleoside the site of the phosphate-ester bond. Structure of purines. Purine biosynthesis is complex.

Acid phosphatase hydrolyses phosphate ester bonds under an optimum pH of 4.8 and an optimum temperature of 37 °C. In this experiment you will use an artificial substrate, para-nitrophenylphosphate ...