为解决遗传性血色素沉着症（HH）在植入前遗传学检测（PGT）中依赖间接单倍型分析的局限性，研究人员开发了一种基于聚合酶链反应-限制性片段长度多态性（PCR-RFLP）的直接检测方法。该研究成功验证了针对HFE基因C282Y变体的检测体系，在80例细胞系、38例患者 ...

为解决埃及儿童非综合征性感音神经性耳聋（SNHL）的遗传病因学问题，埃及国家研究中心的Hala M. Zeidan团队开展了线粒体12S rRNA A1555G突变筛查研究。通过PCR-RFLP技术对30例患儿进行检测，发现仅1例（3.3%）携带该突变，提示其在埃及人群中的贡献有限。该研究为中东地区线粒体耳聋突变谱提供了新数据，对预防氨基糖苷类抗生素耳毒性具有临床警示意义。

PCR-RFLP allows rapid detection of point mutations after the genomic sequences are amplified by PCR. The mutation is discriminated by digestion with specific restriction endonucleases and is ...

One of these mutations was considered to be CYP2C19*3 by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). This result suggests that mutations classed as CYP2C19*3 ...

However, it has become almost obsolete with the advent of relatively simple and less expensive DNA profiling technologies such as the polymerase chain reaction (PCR). The RFLP procedure requires ...

Nucleic acid analysis, genotyping, PCR, and RFLP methodologies are time-consuming techniques using agarose or acrylamide gel electrophoresis—image capture plus gel image interpretations.

Genetic and proteomic features associated with survival after treatment with erlotinib in first-line therapy of non-small cell lung cancer No significant financial relationships to disclose. This is ...

The Indian Council of Medical Research (ICMR) will soon commercialise its newly developed technology on Novel Primers for a PCR-RFLP assay for accurate identification of pathogenic mycobacteria at ...

This software quantitatively analyzes PCR, Northern, Southern, and Western Blots, as well as Restriction Fragment Length Polymorphism. The original Gel-Pro Analyzer was designed to automate gel ...