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PCR for the fragments was conducted according ... Minisequencing primers design and primer-extension reactions for DHPLC The sequence of the minisequencing extension primer used in this study ...
The DHPLC system used to analyze non-fluorescently labeled PCR products was a Transgenomic Wave Nucleic Acid Fragment Analysis System (Transgenomic, San Jose, CA). DHPLC was carried out on ...
This assay combines PCR and DHPLC and can be used to conduct efficient genotyping of the human population, which in turn will help in the diagnosis and treatment of Alzheimer's disease.
Alternatively, DHPLC approach reveals the presence of genetic variation by the differential retention of the homo- and heteroduplex DNA molecules on ion-pair reversed-phase high-pressure liquid ...