Once scientists have zeroed in on a specific segment of DNA, how do they produce enough copies of that segment for their research? In most cases, the polymerase chain reaction, or PCR, is their ...

She earned her PhD in biomedical sciences at the University of Texas Health Science Center, with a focus on cancer biology. She is passionate about making complex scientific concepts accessible to ...

In particular, real-time reverse transcription (RT)-PCR has become the method of choice to rapidly and quantitatively examine the expression of specific genes. Scientists can readily detect as ...

That said, running PCR successfully involves understanding how the technique works and how different parameters affect the reaction itself and the data generated. Furthermore, scientists have ...

This technique allows scientists to see the results almost immediately while the process is still ongoing, whereas conventional RT–PCR only provides results at the end of the process. Real time RT–PCR ...

ABL1 digital PCR can reliably quantify stable deep molecular remission of chronic myeloid leukemia (CML), which will help to determine for which patients chronic drug treatment could potentially be ...

Surgery may not be the best next course of treatment for patients with early-stage breast cancer who had a complete response ...

PCR-Machines are based on Polymerase Chain Reaction (PCR) and produce copies of genes exponentially. The PCR cycling reactions are carried out in an automated thermocycler, which is capable of ...

This is quantitative reverse transcription PCR (RT-qPCR), which means that this extra step was needed before PCR. With PCR, scientists compare a sample taken from a suspected patient, say, of saliva, ...

ABL1 digital PCR can reliably quantify stable deep molecular remission of chronic myeloid leukemia (CML), which will help to determine for which patients chronic drug treatment could potentially be ...

These integrations have the potential to revolutionize fields such as personalized medicine and infectious disease detection, pushing the boundaries of what's possible in modern science. In the first ...