Pipet the emulsion into the wells of a PCR plate as 10 aliquots of 50 μl. Pipet 50 μl of the aqueous phase (Step 3) into a well as a nonemulsified control. Overlay the emulsified and ...
The Applied Biosystems QuantStudio Absolute Q Digital PCR System is a plate-based digital PCR (dPCR) platform powered by proprietary microfluidic array plate (MAP) technology. This allows all the ...