Heat-sensitive chemical modifications to the enzyme's active site can also have a similar effect. A typical PCR mix also contains a variety of other components that play an important role in ensuring ...

1| Combine the following components of the master mix on ice and mix thoroughly ... Note, the pH is 9.2 (the optimal for long PCR) without adjustment; some vectors may require pH 7.2. 10-40 ...

Figure 1: Amplification of complex gene libraries by conventional PCR and emulsion PCR ... should be performed at 25 °C using 25 °C components for the main protocol and on ice using ice-cold ...