The 10–kb, PCR–amplified fragment was detected by gel electrophoresis as a chief band (Fig. 2). The correctly assembled DNA was a chief species and could be easily separated by electrophoresis ...

At the completion of the reaction, amplification products are analyzed by size fractionation using gel electrophoresis. Real-time PCR follows the same course, except that the products are detected ...

Extension: Nucleotides are added to the primers, which serve as extension points to continue new strand synthesis. Endpoint analysis: Scientists analyze PCR products typically using agarose gel ...

At first, they could not determine if they achieved specific target amplification; their gel electrophoresis results were also large smears. They needed to determine if their product was part of this ...

DNA extracted using the PureFood Kit can be used directly in a variety of downstream applications such as real-time PCR, gel electrophoresis, microarrays, and sequencing (e.g., NGS and Sanger).