1. Prepare a 40 ml 1 % agarose gel in 0.5X TBE, containing 0.5 µg/ml ethidium bromide. Each gel will be shared by 2 groups on the same bench. 3. Each group will load PCR and control samples on one ...
Note: An expanded version of this article, with complete Methods and Materials ... was calculated from a set of PCR calibrators included in each experiment. When using RT-PCR for quantitation ...