If nitrocellulose is being used, the membrane must then be baked for 2 hours at 80°C to fix the DNA to the filter. This is not necessary for nylon, which forms covalent bonds with nucleic acids under ...

This protocol describes the transfer of RNA from agarose gels to nylon membranes and the fixation of the RNA to the membrane 1,2,3. You have full access to this article via your institution.