The possibility of using DTT during the purification procedure constitutes an important advantage of calmodulin-affinity chromatography over the most popular nickel ... column with 2.5 ml ...

The steps involved lysis, DEAE–sepharose chromatography ... at a pH of 6.5. The incubation mixture was passed through a nickel column. The column was washed with 50 mM acetate buffer, pH ...

“But nickel column affinity chromatography is a well-established method, so our customers use the antibody mostly for detection” Steenstra notes. A benefit of polyhistidine-specific antibodies ...