We used engineered CRISPR (clustered ... accurately this qPCR method estimates mutational efficiency, we directly compared it to NGS analysis across eight independent screen targets.

过去15年来，基因编辑技术CRISPR-Cas9的进步，使人们对特定基因在许多疾病中发挥的作用有了重要的新见解。但是到目前为止，这项允许科学家使用“引导”RNA来修改DNA序列并评估其效果的技术只能用单个引导RNA靶向、删除、替换或修改单个基因序列，并且同时 ...

Researchers at Novartis BioMedical Research in the US and Switzerland have identified a molecular target that may provide a ...

The study has now been published in Nature ... CRISPR screen, it often takes months to precisely separate the cells according to their function." Another advantage of the new method is that ...

More information: Paul Datlinger et al, Pooled CRISPR screening with single-cell transcriptome readout, Nature Methods (2017). DOI: 10.1038/nmeth.4177 ...

A new, precision-targeted delivery method for CRISPR-Cas9, published Jan. 11 in the journal Nature Biotechnology, enables gene editing on very specific subsets of cells while still in the body - a ...

demonstrated a new CRISPR method that can modify dozens of genes simultaneously, allowing for more large-scale cell reprogramming. In a recently published paper in the journal Nature Methods ...

But a new CRISPR screening method developed at Scripps Research has the potential to uncover new therapeutic targets and treatments for these conditions. The method, outlined in a study published ...

When injected into the organism, this bubble binds to and fuses with the cellular membrane, which pushes the CRISPR system into the cell itself. The method also uses a Cas9 protein itself ...