Carrying out amplification at the end of the process, once molecules are physically separated on the microarray, reduces competition and other effects. Optimally, a process should detect a single ...

Two-color hybridization allows comparison of two samples labeled with different fluorescent dyes on the same microarray ... probes than cDNA from any single cell line. Ten different cell lines ...

One way they do this is to use a DNA microarray to determine the expression ... is isolated from the samples. The mRNA is color-coded with fluorescent tags and used to make a DNA copy (the mRNA ...

The detection rates vary between microarray platforms because of differences in probe density, targeted and backbone coverage. One study demonstrated that reduction in the backbone density reduced ...