This article discusses the most common protein quantification methods that are absorbance at 280 nm, Bradford, Bicin-Choninic Acid (BCA), and Lowry assays. The UV light is absorbed at 280 nm ...

Assay reagents include dye-protein binding, such as Coomassie/Bradford reagents, or copper chelation, as in Lowry and Bicinchoninic Acid (BCA). Each method has its advantages depending on the time ...

This leads to a robust and validated method for protein quantification. The validation shows the main advantages of this quantification method compared to other assays like Bradford or Lowry ...

Total protein concentration can be determined using Bradford, bicinchonic acid (BCA) and Lowry assays, although the composition of control proteins used to draw the standard curve should closely ...

1 Determination of the presence or absence of the 14-3-3 protein in the CSF is sometimes confusing because of equivocal bands, which can complicate the interpretation of assay results. Therefore ...