This article discusses the most common protein quantification methods that are absorbance at 280 nm, Bradford, Bicin-Choninic Acid (BCA), and Lowry assays. The UV light is absorbed at 280 nm ...

Assay reagents include dye-protein binding, such as Coomassie/Bradford reagents, or copper chelation, as in Lowry and Bicinchoninic Acid (BCA). Each method has its advantages depending on the time ...

This leads to a robust and validated method for protein quantification. The validation shows the main advantages of this quantification method compared to other assays like Bradford or Lowry ...